| # | Song | Film | Year | Performer(s) | Song writer(s) | 
| 1 | "Over the Rainbow" | The Wizard of Oz | 1939 | Judy Garland | Harold Arlen, E.Y. Harburg | 
| 2 | "As Time Goes By" | Casablanca | 1942 | Dooley Wilson | Herman Hupfeld | 
| 3 | "Singin' in the Rain" | Singin' in the Rain | 1952 | Gene Kelly | Arthur Freed, Nacio Herb Brown | 
| 4 | "Moon River" | Breakfast at Tiffany's | 1961 | Audrey Hepburn | Henry Mancini, Johnny Mercer | 
| 5 | "White Christmas" | Holiday Inn | 1942 | Bing Crosby | Irving Berlin | 
| 6 | "Mrs. Robinson" | The Graduate | 1967 | Simon & Garfunkel | Paul Simon | 
| 7 | "When You Wish upon a Star" | Pinocchio | 1940 | Cliff Edwards aka "Ukulele Ike"  (as Jiminy Cricket) | Leigh Harline, Ned Washington | 
| 8 | "The Way We Were" | The Way We Were | 1973 | Barbra Streisand | Alan Bergman, Marilyn Bergman, Marvin Hamlisch | 
| 9 | "Stayin' Alive" | Saturday Night Fever | 1977 | The Bee Gees | Barry Gibb, Robin Gibb, Maurice Gibb | 
| 10 | "The Sound of Music" | The Sound of Music | 1965 | Julie Andrews | Richard Rodgers, Oscar Hammerstein II | 
| 11 | "The Man That Got Away" | A Star Is Born | 1954 | Judy Garland | Harold Arlen, Ira Gershwin | 
| 12 | "Diamonds Are a Girl's Best Friend" | Gentlemen Prefer Blondes | 1953 | Marilyn Monroe | Jule Styne,  Leo Robin | 
| 13 | "People" | Funny Girl | 1968 | Barbra Streisand | Jule Styne, Bob Merrill | 
| 14 | "My Heart Will Go On" | Titanic | 1997 | Celine Dion | James Horner, Will Jennings | 
| 15 | "Cheek to Cheek" | Top Hat | 1935 | Fred Astaire, Ginger Rogers | Irving Berlin | 
| 16 | "Evergreen (Love Theme from A Star Is Born)" | A Star Is Born | 1976 | Barbra Streisand | Barbra Streisand, Paul Williams | 
| 17 | "I Could Have Danced All Night" | My Fair Lady | 1964 | Marni Nixon (dubbing Audrey Hepburn) | Frederick Loewe, Alan Jay Lerner | 
| 18 | "Cabaret" | Cabaret | 1972 | Liza Minnelli | John Kander, Fred Ebb | 
| 19 | "Someday My Prince Will Come" | Snow White and the Seven Dwarfs | 1937 | Adriana Caselotti (as Snow White) | Larry Morey, Frank Churchill | 
| 20 | "Somewhere" | West Side Story | 1961 | Jimmy Bryant (dubbing Richard Beymer), Marni Nixon (dubbing Natalie Wood) | Leonard Bernstein, Stephen Sondheim | 
| 21 | "Jailhouse Rock" | Jailhouse Rock | 1957 | Elvis Presley | Jerry Leiber, Mike Stoller | 
| 22 | "Everybody's Talkin'" | Midnight Cowboy | 1969 | Harry Nilsson | Fred Neil | 
| 23 | "Raindrops Keep Fallin' on My Head" | Butch Cassidy and the Sundance Kid | 1969 | B.J. Thomas | Hal David, Burt Bacharach, Luther Dixon | 
| 24 | "Ol' Man River" | Show Boat | 1936 | Paul Robeson | Jerome Kern, Oscar Hammerstein II | 
| 25 | "High Noon (Do Not Forsake Me, Oh My Darlin')" | High Noon | 1952 | Tex Ritter | Dimitri Tiomkin, Ned Washington | 
| 26 | "The Trolley Song" | Meet Me in St. Louis | 1944 | Judy Garland | Hugh Martin, Ralph Blane | 
| 27 | "Unchained Melody" | Ghost | 1990 | The Righteous Brothers | Alex North, Hy Zaret | 
| 28 | "Some Enchanted Evening" | South Pacific | 1958 | Giorgio Tozzi (dubbing Rossano Brazzi) | Richard Rodgers, Oscar Hammerstein II | 
| 29 | "Born to Be Wild" | Easy Rider | 1969 | Steppenwolf | Mars Bonfire | 
| 30 | "Stormy Weather" | Stormy Weather | 1943 | Lena Horne | Harold Arlen, Ted Koehler | 
| 31 | "Theme from New York, New York" | New York, New York | 1977 | Liza Minnelli | John Kander, Fred Ebb | 
| 32 | "I Got Rhythm" | An American in Paris | 1951 | Gene Kelly | George Gershwin, Ira Gershwin | 
| 33 | "Aquarius" | Hair | 1979 | Ren Woods, Ensemble | James Rado, Gerome Ragni, Galt MacDermot | 
| 34 | "Let's Call the Whole Thing Off" | Shall We Dance | 1937 | Fred Astaire, Ginger Rogers | George Gershwin, Ira Gershwin | 
| 35 | "America" | West Side Story | 1961 | Rita Moreno, George Chakiris, Ensemble | Stephen Sondheim, Leonard Bernstein | 
| 36 | "Supercalifragilisticexpialidocious" | Mary Poppins | 1964 | Julie Andrews, Dick Van Dyke, Ensemble | The Sherman Brothers | 
| 37 | "Swinging on a Star" | Going My Way | 1944 | Bing Crosby | Jimmy Van Heusen, Johnny Burke | 
| 38 | "Theme from Shaft" | Shaft | 1971 | Isaac Hayes, Chorus | Isaac Hayes | 
| 39 | "Days of Wine and Roses" | Days of Wine and Roses | 1962 | Chorus | Henry Mancini, Johnny Mercer | 
| 40 | "Fight the Power" | Do the Right Thing | 1989 | Public Enemy | Carlton Ridenhour, Eric Sadler, Hank Saxley, Keith Boxley | 
| 41 | "New York, New York" | On the Town | 1949 | Gene Kelly, Frank Sinatra, and Jules Munshin | Leonard Bernstein, Betty Comden, Adolph Green | 
| 42 | "Luck Be a Lady" | Guys and Dolls | 1955 | Marlon Brando, Ensemble | Frank Loesser | 
| 43 | "The Way You Look Tonight" | Swing Time | 1936 | Fred Astaire | Jerome Kern, Dorothy Fields | 
| 44 | "Wind Beneath My Wings" | Beaches | 1988 | Bette Midler | Jeff Silbar, Larry Henley | 
| 45 | "That's Entertainment" | The Band Wagon | 1953 | Fred Astaire, Jack Buchanan, Nanette Fabray, Oscar Levant | Arthur Schwartz, Howard Dietz | 
| 46 | "Don't Rain on My Parade" | Funny Girl | 1968 | Barbra Streisand | Bob Merrill, Jule Styne | 
| 47 | "Zip-a-Dee-Doo-Dah" | Song of the South | 1947 | James Baskett | Allie Wrubel, Ray Gilbert | 
| 48 | "Que Sera, Sera (Whatever Will Be, Will Be)" | The Man Who Knew Too Much | 1956 | Doris Day | Jay Livingston, Ray Evans | 
| 49 | "Make 'Em Laugh" | Singin' in the Rain | 1952 | Donald O'Connor | Nacio Herb Brown, Arthur Freed | 
| 50 | "Rock Around the Clock" | Blackboard Jungle | 1955 | Bill Haley and His Comets | Max C. Freedman, James E. Myers | 
| 51 | "Fame" | Fame | 1980 | Irene Cara | Michael Gore, Dean Pitchford | 
| 52 | "Summertime" | Porgy and Bess | 1959 | Loulie Jean Norman | George Gershwin, DuBose Heyward | 
| 53 | "Goldfinger" | Goldfinger | 1964 | Shirley Bassey | Leslie Bricusse, Anthony Newley, John Barry (composer) | 
| 54 | "Shall We Dance?" | The King and I | 1956 | Marni Nixon (dubbing Deborah Kerr), Yul Brynner | Richard Rodgers, Oscar Hammerstein II | 
| 55 | "Flashdance... What a Feeling" | Flashdance | 1983 | Irene Cara | Giorgio Moroder, Keith Forsey, Irene Cara | 
| 56 | "Thank Heaven for Little Girls" | Gigi | 1958 | Maurice Chevalier | Alan Jay Lerner, Frederick Loewe | 
| 57 | "The Windmills of Your Mind" | The Thomas Crown Affair | 1968 | Noel Harrison | Alan Bergman, Marilyn Bergman, Michel Legrand | 
| 58 | "Gonna Fly Now" | Rocky | 1976 | DeEtta Little, Nelson Pigford | Bill Conti, Carol Connors, Ayn Robbins | 
| 59 | "Tonight" | West Side Story | 1961 | Marni Nixon (dubbing Natalie Wood), Jimmy Bryant (dubbing Richard Beymer) | Leonard Bernstein, Stephen Sondheim | 
| 60 | "It Had to Be You" | When Harry Met Sally... | 1989 | Frank Sinatra, Harry Connick Jr. | Isham Jones, Gus Kahn | 
| 61 | "Get Happy" | Summer Stock | 1950 | Judy Garland | Harold Arlen, Ted Koehler | 
| 62 | "Beauty and the Beast" | Beauty and the Beast | 1991 | Angela Lansbury (as Mrs. Potts) | Howard Ashman, Alan Menken | 
| 63 | "Thanks for the Memory" | The Big Broadcast of 1938 | 1938 | Bob Hope, Shirley Ross | Ralph Rainger, Leo Robin | 
| 64 | "My Favorite Things" | The Sound of Music | 1965 | Julie Andrews | Richard Rodgers, Oscar Hammerstein II | 
| 65 | "I Will Always Love You" | The Bodyguard | 1992 | Whitney Houston | Dolly Parton | 
| 66 | "Suicide Is Painless" | MASH | 1970 | Chorus | Johnny Mandel, Mike Altman | 
| 67 | "Nobody Does It Better" | The Spy Who Loved Me | 1977 | Carly Simon | Carol Bayer Sager, Marvin Hamlisch | 
| 68 | "Streets of Philadelphia" | Philadelphia | 1993 | Bruce Springsteen | Bruce Springsteen | 
| 69 | "On the Good Ship Lollipop" | Bright Eyes | 1934 | Shirley Temple | Richard A. Whiting, Sidney Clare | 
| 70 | "Summer Nights" | Grease | 1978 | John Travolta, Olivia Newton-John | Jim Jacobs, Warren Casey | 
| 71 | "(I'm a) Yankee Doodle Dandy" | Yankee Doodle Dandy | 1942 | James Cagney | George M. Cohan | 
| 72 | "Good Morning" | Singin' in the Rain | 1952 | Gene Kelly, Donald O'Connor, Debbie Reynolds | Arthur Freed, Nacio Herb Brown | 
| 73 | "Isn't It Romantic?" | Love Me Tonight | 1932 | Maurice Chevalier, Jeanette MacDonald | Richard Rodgers, Lorenz Hart | 
| 74 | "Rainbow Connection" | The Muppet Movie | 1979 | Jim Henson (as Kermit the Frog) | Paul Williams, Kenneth Ascher | 
| 75 | "Up Where We Belong" | An Officer and a Gentleman | 1982 | Joe Cocker, Jennifer Warnes | Will Jennings, Jack Nitzsche, Buffy Sainte-Marie | 
| 76 | "Have Yourself a Merry Little Christmas" | Meet Me in St. Louis | 1944 | Judy Garland | Hugh Martin, Ralph Blaine | 
| 77 | "The Shadow of Your Smile" | The Sandpiper | 1965 | Chorus | Johnny Mandel, Paul Francis Webster | 
| 78 | "9 to 5" | Nine to Five | 1980 | Dolly Parton | Dolly Parton | 
| 79 | "Arthur's Theme (Best That You Can Do)" | Arthur | 1981 | Christopher Cross | Christopher Cross, Burt Bacharach, Carol Bayer Sager, Peter Allen | 
| 80 | "Springtime for Hitler" | The Producers | 1968 | Ensemble | Mel Brooks | 
| 81 | "I'm Easy" | Nashville | 1975 | Keith Carradine | Keith Carradine | 
| 82 | "Ding-Dong! The Witch Is Dead" | The Wizard of Oz | 1939 | Ensemble | Harold Arlen, E.Y. Harburg | 
| 83 | "The Rose" | The Rose | 1979 | Bette Midler | Amanda McBroom | 
| 84 | "Put the Blame on Mame" | Gilda | 1946 | Anita Ellis (dubbing Rita Hayworth) | Allan Roberts, Doris Fisher | 
| 85 | "Come What May" | Moulin Rouge! | 2001 | Nicole Kidman, Ewan McGregor | David Baerwald | 
| 86 | "(I've Had) The Time of My Life" | Dirty Dancing | 1987 | Bill Medley, Jennifer Warnes | Franke Previte, Donald Markowitz, John DeNicola | 
| 87 | "Buttons and Bows" | The Paleface | 1948 | Bob Hope | Jay Livingston, Ray Evans | 
| 88 | "Do Re Mi" | The Sound of Music | 1965 | Julie Andrews, Ensemble | Richard Rodgers, Oscar Hammerstein II | 
| 89 | "Puttin' On the Ritz" | Young Frankenstein | 1974 | Gene Wilder, Peter Boyle | Irving Berlin | 
| 90 | "Seems Like Old Times" | Annie Hall | 1977 | Diane Keaton | Carmen Lombardo, John Jacob Loeb | 
| 91 | "Let the River Run" | Working Girl | 1988 | Carly Simon | Carly Simon | 
| 92 | "Long Ago (and Far Away)" | Cover Girl | 1944 | Gene Kelly, Martha Mears (dubbing Rita Hayworth) | Ira Gershwin, Jerome Kern | 
| 93 | "Lose Yourself" | 8 Mile | 2002 | Eminem | Eminem, Luis Resto, Jeff Bass | 
| 94 | "Ain't Too Proud to Beg" | The Big Chill | 1983 | Temptations | Norman Whitfield, Edward Holland, Jr. | 
| 95 | "(We're Off on the) Road to Morocco" | Road to Morocco | 1942 | Bing Crosby, Bob Hope | Jimmy Van Heusen, Johnny Burke (lyricist) | 
| 96 | "Footloose" | Footloose | 1984 | Kenny Loggins | Kenny Loggins, Dean Pitchford | 
| 97 | "42nd Street" | 42nd Street | 1933 | Ruby Keeler, Dick Powell, Ensemble | Harry Warren, Al Dubin | 
| 98 | "All That Jazz" | Chicago | 2002 | Catherine Zeta-Jones, Renée Zellweger | John Kander, Fred Ebb | 
| 99 | "Hakuna Matata" | The Lion King | 1994 | Nathan Lane, Ernie Sabella, Jason Weaver, Joseph Williams | Elton John, Tim Rice | 
| 100 | "Old Time Rock and Roll" | Risky Business | 1983 | Bob Seger | George Jackson, Thomas E. Jones III |